Title: The Biological bulletin
Identifier: biologicalbullet177mari (find matches)
Year: [1] (s)
Authors: Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983
Subjects: Biology; Zoology; Biology; Marine Biology
Publisher: Woods Hole, Mass. : Marine Biological Laboratory
Contributing Library: MBLWHOI Library
Digitizing Sponsor: MBLWHOI Library

View Book Page: Book Viewer
About This Book: Catalog Entry
View All Images: All Images From Book
Click here to view book online to see this illustration in context in a browseable online version of this book.

Text Appearing Before Image:
LIFE HISTORY OF P. JUGOSUS 117
Text Appearing After Image:
Figure 5. Phase/fluorescence micrographs of DAPI-stained amoebae. A,B. Amoeba with metaphase nucleus. Phase micrograph shows nucleus clearly intact and under fluorescence, DNA is seen condensed in the center. C,D. Amoebae with telophase nucleus. D,F. Cytoplasmic DNA is faintly visible in the amoe- bae. E,F. Arrow indicates a structure which may be a chromatin body extruded by the amoeba. Its size and fluorescent properties differ from cytoplasmic fluorescence. Bar scale = 10 ^m. nonspecific brightly staining material in the preparations. Unstained amoeba slide preparations were also used as controls to detect and distinguish autofluorescence from authentic DNA staining. Excitation filter sets on the Ni- kon Fluophot in the UV and blue (approximately 365 nm and 490 nm) were used for epifluorescence observations of DAPI and mithramycin samples, respectively. Photo- micrographs were made with a Nikon Microflex AFX photomicrographic attachment. Films used for photomi- croscopy were Scotch 640 ASA tungsten film and Kodak Tri-X 400 ASA pushed to 1600. Electron microscopy Fixation and embedding of the Cuba strain of P. jugo- sus. Amoebae inoculated into aqueous suspension (de-

Note About Images