File:The schematic diagram of CRISPR-Cas9.webp
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Summary
| Description |
English: The widespread application of gene editing technology in crop improvement at present largely benefits from the emergence of the CRISPR/Cas9 system, which is derived from the immune system of bacteria and archaea. The CRISPR/Cas9 system was synthesized by connecting single-guide RNA (sgRNA) and Cas9. The sgRNA can bind to the target site after specific modification. The RuvC domain of Cas9 protein can cleave the DNA strand with protospacer-associated motif (PAM) sites, and the DNA strand complementary to sgRNA is cleaved by the HNH domain of Cas9 protein. The cutting site is near the PAM site. |
| Date | |
| Source | https://www.mdpi.com/2073-4395/12/4/759 |
| Author | Ye, Runle, Xi Yang, and Yuchun Rao |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 22:48, 28 December 2023 | | 2,529 × 1,107 (542 KB) | wikimediacommons>DoctorNaturopath | Uploaded a work by Ye, Runle, Xi Yang, and Yuchun Rao from https://www.mdpi.com/2073-4395/12/4/759 with UploadWizard |
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